Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation

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Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation.

Chloroplasts were separated from Euglena gracilis by zonal centrifugation at low speed in density gradients of Ficoll or dextran. The chloroplasts were intact by the criteria of ultrastructure and their content of ribulose diphosphate carboxylase and soluble protein. The chloroplasts also contained ribosomes and ribosomal RNA uncontaminated by the corresponding cytoplasmic particles.

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1971

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.47.2.217